The Scientist 2018 12.pdf

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DECEMBER 2018 | WWW.THE-SCIENTIST.COM
INVISIBLE
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MYSTERIOUS BACTERIAL
MICROCOMPARTMENTS
A NOBELIST’S RACE
FOR THE RIBOSOME
IMAGING CHROMATIN
AN EMERGING APPRECIATION FOR MEMBRANELESS
ORGANELLES AND THE LIQUID DYNAMICS THAT SHAPE THEM
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10 INNOVATIONS
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5–10 TPM
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7000
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0
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1
2
0
1
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Sequencing libraries were generated from Jurkat single cells (6 replicates) using the NEBNext
Single Cell/Low Input RNA Library Prep Kit, or the SMART-Seq
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v4 Ultra
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Low Input RNA
Kit for Sequencing plus the Nextera
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XT DNA Library Prep Kit. Libraries were sequenced
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500. Each dot represents the number of transcripts identified at the
given Transcripts Per Kilobase Million (TPM) range, and each box represents the median,
first and third quartiles per replicate and method. Salmon 0.6 was used for read mapping and
quantification of all GENCODE v25 transcripts. Increased identification of low abundance
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© Copyright 2018, New England Biolabs, Inc.; all rights reserved.
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DECEMBER 2018
Contents
THE SCIENTIST
THE-SCIENTIST.COM
VOLUME 32 NUMBER 12
© MARK MAWSON, GETTY IMAGES; © THOM GRAVES; © ISTOCK.COM, VENIMO
Features
ON THE COVER: © ABSTRACT OIL WORK, ALAMY STOCK PHOTO
28
Cellular Droplets
Newly discovered liquid dynamics
within the cytoplasm and nucleus play
critical roles in cell biology.
BY TIM NOTT AND MICHAEL CRABTREE
36
Shell Games
Bacteria, traditionally thought to lack
organelles, get a metabolic boost from
geometric compartments that act
as cauldrons for chemical reactions.
BY AMBER DANCE
44
Top 10 Innovations
Biology happens on many levels, from
ecosystems to electron transport chains.
These tools may help spur discoveries
at all of life’s scales.
BY
THE SCIENTIST
STAFF
12. 2 01 8 | T HE S C IEN T IST
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